ression degree of CSNK2A1 was linked to poor prognosis of OS for LIHC in TCGA dataset. Following this, we performed a retrospective clinical study on our own individuals to decide regardless of whether the expression degree of CSNK2A1 could predict the prognosis of sufferers with LIHC. Survival analysis from the 48 clinical LIHC sufferers was conducted. As shown in Figure 7E, LIHC patients with high levels of protein expression of CSNK2A1 in tumor tissues had considerably shorter OS occasions in comparison to these withValidation of the CSNK2A1 Expression Levels in Clinical Tissue Samples from LIHC Sufferers of SYSUCC CohortIHC staining comparison of CSNK2A1 expression status amongst tumor tissues and adjacent regular tissues from LIHC patients was performed to validate earlier bioinformatic outcomes. Based on the outcomes of IHC-P score of CSNK2A1 in tumor tissues from SYSUCC cohort, we could divide SYSUCC cohort (48 circumstances) into high CSNK2A1-doi.org/10.2147/IJGM.SBax Gene ID International Journal of Common Medicine 2021:DovePressPowered by TCPDF (tcpdf.org)DovepressWu et alFigure five Correlation of CSNK2A1 expression with immune infiltration level in TCGA cancers. (A) Correlation of CSNK2A1 expression with infiltration amount of 22 types of immune cell subtypes in TCGA. Immune cells positively correlating with CSNK2A1 expression in TCGA database had been labeled in red, though immune cells negatively correlating with CSNK2A1 expression in TCGA database had been labeled in yellow. (B) Correlation of CSNK2A1 expression with immune infiltration level in BRCA, PRAD and UCEC. CSNK2A1 expression had important good correlation with resting CD4+ T memory cells and M1-macrophages, and important negative correlation with CD8+ T cells, monocytes, activated NK cells and plasma cells. P0.05; P0.01; P0.001. Abbreviations: CSNK2A1, casein kinase two alpha protein 1; TCGA, the cancer genome atlas; Neu, neutrophils; Eos, eosinophils; act-Mas, activated mast cells; res-Mas, resting mast cells; act-DC, activated dendritic cells; res-DC, resting dendritic cells; Mac-M2, macrophages M2; Mac-M1, macrophages M1; Mac-M0, macrophages M0; Mono, monocytes; act-NK, activated NK cells; DDR1 Synonyms res-NK, resting NK cells; -T, -T cells; Tregs, regulatory T cells; follic-Th, follicular T helper cells; act-CD4+T, activated CD4+ T memory cells; res-CD4+T, resting CD4+ T memory cells; naivCD4+T, naive CD4+ T cells; CD8+T, CD8+ T cells; memo-B, memory B cells; naivB, naive B cells; BRCA, breast invasive carcinoma; PRAD, prostate adenocarcinoma; UCEC, uterine corpus endometrial carcinoma.International Journal of General Medicine 2021:doi.org/10.2147/IJGM.SDovePressPowered by TCPDF (tcpdf.org)Wu et alDovepressFigure 6 Correlations in between CSNK2A1 expression and tumor immunotherapy biomarkers in TCGA cancers. (A) Correlation amongst CSNK2A1 expression and immune checkpoint marker sets. (B) Radar map of correlation in between CSNK2A1 expression and TMB. (C) Radar map of correlation involving CSNK2A1 expression and MSI. P0.05; P0.01; P0.001. Abbreviations: CSNK2A1, casein kinase 2 alpha protein 1; TCGA, the cancer genome atlas; TMB, tumor mutational burden; MSI, microsatellite instability.low expression levels of CSNK2A1 (P = 0.017), which was constant with our preceding bioinformatic final results in LIHC situations from TCGA dataset.PPI Network and Enrichment Analysis of CSNK2A1 in CancersNext, to discover the prospective roles that CSNK2A1 took portion inside the carcinogensis of cancers, we employed GeneMANIA on-line tool to receive a PPI network for CSNK