M formation and higher enzyme production is not well understood at the molecular level. In this context, here we illustrate a summary classification and a set of the* Correspondence: [email protected] ^Deceased 1 Laboratorio de Micolog y Biotecnolog , Universidad Nacional Agraria La Molina, Av. La Molina s/n, 12 Lima, Peru Full list of author information is available at the end of the articlefeatures of A. niger strain ATCC 10864 with a high-quality draft genome sequence description and annotation.Organism informationClassification and featuresAspergillus niger strain ATCC 10864 is a haploid, filamentous, black ornamented asexual spore (conidia) producing fungi belonging to the order Eurotiales and family Trichocomaceae (Fig. 1) and probably originated in Budapest, Hungary [9]. It is most commonly found in mesophilic environments such as decaying vegetation or soil with growth temperature from 6 ?7 [10] and optimal growth at 25-35 [11] as well as a wide pH range: 1.4-9.8 (Table 1). Alvocidib web Hyphae of A. niger ATCC 10864 are septate, hyaline and the conidiophores are long, smooth-walled, hyaline, becoming darker at the apex and ending in a globose to subglobose vesicle [12]. Phylogenetic analysis was performed by the maximum likelihood method based on 18S rRNA gene sequences and the analysis revealed close relationship of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28298493 our strain with other type strains of A. niger (Fig. 2). Although the genomes of three A. niger type strains (CBS 513.88, ATCC 1015 and SH2) have already been sequenced [13?5], biofilm?The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Paul et al. Standards in Genomic Sciences (2017) 12:Page 2 ofFig. 1 Electron micrograph of Aspergillus niger strain ATCC 10864. Inset: ornamented sporesforming and high productive strain of A. niger such as ATCC 10864 is still being neglected and only very few sequence information are available in the databases.Genome sequencing informationGenome project historyExperimental studies with A. niger ATCC 10864 have provided four reasons to select this strain for whole genome sequencing: 1) This is the first reported biofilm forming A. niger strain [16]. 2) Biofilm culture of this strain can produce 2-3 times more lignocellulolytic enzymes compared to conventional submerged culture [4, 7, 8]. 3) The key mechanism that controls higher levels of enzyme production of the organism in biofilm culture is still unclear. 4) The genomes of only three strains of such an industrially relevant fungus are available in the databases [13?5]. A high-quality draft genome sequence has been deposited both in Genomes On Line Database (GOLD) [17] and DDBJ/ EMBL/GenBank under accession numbers Gp0155299 and MCQH00000000 respectively. Table 2 presents the project information and its association with the minimum information about a genome sequence version 2.0 compliance [18].Growth conditions and genomic DNA preparationof the culture medium in 125 ml flasks was inoculated.

By mPEGS 1