Olume and TP, AP, and Ca2+; whereas volume exhibited a good correlation with GLU. Despite the fact that sperm concentration was negatively correlated withResults Semen CharacteristicsAlthough all bulls produced spermic ejaculates (n = 21 bulls; 205 ejaculates), 55 (113/205) of ejaculates have been not incorporated in theTable 1. Qualities of Asian elephant ejaculates and comparison of semen traits between fantastic and poor ErbB2/HER2 site Motility ejaculates.All Ejaculates (92 ejaculates; 11 bulls) Semen Trait Total volume (ml) Sperm concentration (6106 cells/ml) Mean 98.five 1008.2 6 SD 679.6 6800.8 636.three 635.3 628.6 626.1 662.2 60.8 (Variety) (three?46) (9?665) (0?5) (0?5) (0?five) (0?7) (112?37) (4.9?.3)Very good Motility (28 ejaculates; 5 bulls) Mean 135.1 473.0 81.three 75.two 80.three 51.9 265.4 7.07 six SD 689.a aPoor Motility (52 ejaculates; ten bulls) (Variety) (20?46) (10?000) (65?five)aMean 88.eight 1313.8 1.9 1.two 44.9 7.five 282.eight 6.six SD 673.b b(Range) (7.5?10) (two?665) (0?0) (0?0)b b6511.2 69.a6764.7 63.6 63.b bTotal motility ( tMOT) 31.three Progressive motility ( pMOT) Normal sperm ( ) Spermac good ( ) Osmolality (mOsm) pH 28.0 59.five 24.0 271.7 six.618.eight 613.0 614.five 612.1 60.a(10?5) (47?five) (31?0) (242?91) (6.00?.29)a a630.eight 614.b(2?eight) (0?9) (112?37) (4.89?.81)6103.5 60.Good Motility ejaculates: 65 tMOT; Poor Motility ejaculates: #10 tMOT. a,b Inside a row, suggests with various superscripts between very good versus poor motility ejaculates differ (P,0.05). Ejaculates with overt visual or olfactory signs of urine contamination had been not integrated. doi:ten.1371/journal.pone.0071033.tPLOS A single | plosone.orgLactotransferrin in Elephant Seminal PlasmaTable 2. Summary statistics for seminal plasma components1.All Ejaculates (9 Bulls; Except: 6 Bulls, 4 Bulls) N TP ALB LDH CPK AST ALT AP Ca2+ P32 Na+ K+ Cl2 Mg2+ `Good Motility (five Bulls; Except: four Bulls, 3 Bulls) N 24 23 ten 22 24 24 23 24 24 22 22 22 20 22 12 10 24 six{ Mean 4.67 1.3 6.20 11.86 9.21 3.46 465.65 8.81 2.74 109.64 18.95 107.14 2.22 6.45 10.50 6.60 3.93 85.83 6 SD 64.50 61.1 615.11 613.12 612.47 62.a a {Poor Motility (9 Bulls; Except: `5 Bulls,4 Bulls) N 36 36 21 36 36 36 36 36 36 36 36 36 29 36 11` 25 36 10 Mean 8.23 1.6 33.48 3.97 12.33 5.92 462.08 17.45 7.44 70.47 24.18 80.50 6.66 1.42 13.73 5.48 5.94 103.20 6 SD 63.7 60.9 661.10 69.bMean 6.87 1.52 24.68 7.27 10.98 4.95 500.78 13.87 5.82 85.25 22.03 89.76 4.82 3.44 13.17 5.80 5.06 96.6 SD 64.3 61.0 652.2 611.9 612.2 64.2 6615.9 619.9 65.6 636.4 617.7 641.4 66.2 66.6 612.3 65.6 67.6 678.(Range) (0?0) (0?) (0?90) (0?5) (0?0) (0?8) (3?125) (1?8) (0?1) (10?50) (3?4) (14?50) (0?2) (0?7) (0?1) (0?2) (0?6) (13?88)(Range) (0?0) (0?) (0?9) (0?1) (0?9) (0?0) (3?125) (1?0) (0?9)a(Range) (10?5) (0?.0) (0?90) (0?5) (0?0) (0?8) (3?688) (2?8) (0?8)b61 60 31 59 61 61 60 61 61 59 59 59 50 59 24` 35 61 16612.23 64.b6701.39 617.36 63.a6435.24 621.20 65.b628.11 618.84 627.36 62.97 69.a a(21?29) (3?4)633.43 617.06 645.19 67.20 62.b b(10?50) (9?8)a(38?35) (0?3) (0?7) (0?1) (3?0) (0?6) (13?88)b(14?50) (1?2) (0?0) (3?8) (0?2) (0?8) (24?87)GLU CHO HCO32 CRT UUN610.48 63.24 610.03 6113.612.31 66.29 65.44 655.1 Seminal Plasma Components: Total Protein (TP), mg/ml; Albumin (ALB), mg/ml; Lactate dehydrogenase (LDH), U/L; Creatine phosphokinase (CPK), U/L; Aspartate CD38 web aminotransferase (AST), U/L; Alanine aminotransferase (ALT), U/L; Alkaline phosphatase (AP), U/L; Calcium (Ca2+), mg/dl; Phosphorus (P32), mg/dl; Sodium (Na+), mmol/L; Potassium (K+), mmol/L; Chloride (Cl2), mmol/L; Magnesium (Mg2+), mg/dl; Glucose (GLU), mg/dl;.