G hybrid solid-phase extraction (SPE) purification coupled to high-temperature gas chromatography-mass spectrometry (HTGC-MS). Methanol-based hybrid SPE elevated the selective extraction, and HTGC resulted inside a good chromatographic resolution for the separation of lipophilic compounds. The limits of quantification of cholesterol and CEs ranged from 0.2 to 10.0 g/ml, although OHCs and cholesterol precursors ranged from 0.01 to 0.10 g/ml. Linearity as the correlation coefficient was higher than 0.99 together with the exception of cholesteryl laurate, myristate, 2 oleate, and linoleate (r 0.98). The precision ( coefficient of variation) and accuracy ( bias) ranged from 1.1 to 9.8 and from 75.9 to 125.1 , respectively. The all round recoveries of CEs ranged from 26.1 to 64.0 , along with the recoveries of other sterols ranged from 83.eight to 129.3 . The cholesterol signatures showed sex differences in individuals with vasospastic angina and may perhaps associate with 24-reductases. This strategy might be valuable for making clinical diagnoses and for an enhanced understanding of the pathophysiology of vasospastic angina.–Son, H-H., J-Y. Moon, H. S. Search engine optimisation, H. H. Kim, B. C. Chung, and M. H. Choi. High-temperature GC-MS-based serum cholesterol signatures might reveal sex variations in vasospastic angina. J. Lipid Res. 2014. 55: 15562.Supplementary crucial words hybrid solid-phase extraction-precipitation cardiovascular disease hydroxycholesterolsex hormones, and corticosteroids. Its biosynthesis is began from acetyl-CoA (2), which results in the formation of lanosterol, the first cholesterol precursor obtaining a steroidal skeleton, and is additional metabolized in two alternate pathways. One pathway consists of lathosterol and 7-dehydrocholesterol as cholesterol precursors, and desmosterol is the precursor within the other pathway. The cholesterol metabolism in tissues and blood may be described as follows. Initial, acyl-CoA:cholesterol acyltransferase (ACAT) and lecithin:cholesterol acyltransferase (LCAT) are involved within the conversion to cholesteryl esters (CEs) with fatty acids (3, 4). CEs act as a major kind of transporter as plasma lipoproteins, or as storage units as lipid droplets (five).Imidacloprid Second, cholesterol is converted to hydroxycholesterols (OHCs) by enzymatic (hydroxylase, cytochrome P450 families) or nonenzymatic hydroxylations at several positions (6).Plasmin OHCs are well-known as regulators of cholesterol homeostasis (7, eight) (Fig.PMID:23892407 1). Alterations of cholesterol metabolism are associated with vasospastic angina, which is thought of an early manifestation of atherosclerotic vascular disease. The elevated degree of cholesterol can be a diagnostic feature of cardiovascular events, and the enhanced synthesis of CEs can also be connected with atherosclerosis (9). Also, OHCs are linked to a danger of atherosclerosis (ten). In particular, 27-OHC inhibits cardioprotective effects of estrogen by competitive antagonism of estrogen receptor binding within the vasculature (11), in addition to a high plasma degree of 7 -OHC was observed in aCholesterol is an amphipathic lipid that may be an crucial component of cell membranes (1), and it truly is a precursor for conversion to other steroids in the physique, such as progestins,This study was supported by the Korea Institute of Science and Technology Institutional System (Project No. 2E24080), and by the Converging Analysis Center Program by means of the Ministry of Education, Science, and Technology (2013K000427). Author’s Choice–Final version full access. Manuscript received 3 June 2013.