Contaminations, standard procedures were taken.30 The resulting sequences had been then analyzed employing the BioEdit software.31 Before tree construction, the best-fitting substitution model was selected employing jModeltest depending on the Akaike Data Criterion (AIC).32 Consistent with our current outcomes, GTR +I+ was found to become the most beneficial for all datasets.11 Under this model, ML trees were heuristically searched by SPR and NNI perturbation algorithms implemented in PHYML.33 Using the tree files generated, ML tree topology was displayed working with the MEGA5 program.AcknowledgmentsFunding: The study was supported by a grant from NIAID/NIH (5 R01 AI080734-03A).REFERENCE1. Simmonds P, Bukh J, Combet C, Deleage G, Enomoto N, et al. Consensus proposals for a unified technique of nomenclature of hepatitis C virus genotypes. Hepatology. 2005; 42:96273. [PubMed: 16149085] two. Pham VH, Nguyen HD, Ho PT, Banh DV, Pham HL, et al. Quite high prevalence of hepatitis C virus genotype 6 variants in southern Vietnam: large-scale survey determined by sequence determination. Jpn J Infect Dis. 2011; 64:53739. [PubMed: 22116339] three. McOmish F, Yap PL, Dow BC, Follett EA, Seed C, et al. Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey. J Clin Microbiol. 1994; 32:88492. [PubMed: 7913097] 4. Pybus OG, Barnes E, Taggart R, Lemey P, Markov PV, et al. Genetic history of hepatitis C virus in East Asia. J Virol. 2009; 83:1071082. [PubMed: 18971279] five. Simmonds P. Genetic diversity and evolution of hepatitis C virus–15 years on. J Gen Virol. 2004; 85:3173188. [PubMed: 15483230] six. Pybus OG, Charleston MA, Gupta S, Rambaut A, Holmes EC, et al. The epidemic behavior of your hepatitis C virus. Science. 2001; 292:2323325. [PubMed: 11423661]J Clin Virol. Author manuscript; accessible in PMC 2014 August 01.Gu et al.Page7. Pybus OG, Cochrane A, Holmes EC, Simmonds P. The hepatitis C virus epidemic amongst injecting drug customers. Infect Genet Evol.Dexrazoxane 2005; 5:13139.Atoltivimab [PubMed: 15639745] eight.PMID:25105126 Tanaka Y, Hanada K, Mizokami M, Yeo AE, Shih JW, et al. A comparison from the molecular clock of hepatitis C virus within the Usa and Japan predicts that hepatocellular carcinoma incidence in the United states will increase more than the subsequent two decades. Proc Natl Acad Sci U S A. 2002; 99:155845589. [PubMed: 12438687] 9. Goedert JJ, Chen BE, Preiss L, Aledort LM, Rosenberg PS. Reconstruction of your hepatitis C virus epidemic inside the US hemophilia population, 1940-1990. Am J Epidemiol. 2007; 165:1443453. [PubMed: 17379617] 10. Hauri AM, Armstrong GL, Hutin YJ. The international burden of illness attributable to contaminated injections provided in health care settings. Int J STD AIDS. 2004; 15:76. [PubMed: 14769164] 11. Fu Y, Qin W, Cao H, Xu R, Tan Y, et al. HCV 6a prevalence in Guangdong province had the origin from Vietnam and recent dissemination to other regions of China: phylogeographic analyses. PLoS 1. 2012; 7:e28006. [PubMed: 22253686] 12. Fu Y, Wang Y, Xia W, Pybus OG, Qin W, et al. New trends of HCV infection in China revealed by genetic analysis of viral sequences determined from first-time volunteer blood donors. J Viral Hepat. 2011; 18:422. [PubMed: 20196805] 13. Lu L, Nakano T, He Y, Fu Y, Hagedorn CH, et al. Hepatitis C virus genotype distribution in China: predominance of closely connected subtype 1b isolates and existence of new genotype six variants. J Med Virol. 2005; 75:53849. [PubMed: 15714489] 14. Wang Y, Okamoto H, Tsuda F, Nagayama R, Tao QM, et a.